Figure 2 of
Wagner, Mol Vis 2002;
Figure 2. Effects of Phorbol Ester on Interaction of PKCg with Cx43
A: Normal, untransfected N/N1003a cells (1x106 cells/ppt) were immunoprecipitated with anti PKCg antisera (Transduction Labs; 1:1000 dilution) and Western blots were probed with anti Cx43 (Chemicon; 1:1000 dilution). TPA was added to the cells at 200 nM for 60 min prior to immunoprecipitation. Lane 1: No TPA, lane 2: TPA added. B: Western blot from A was rinsed in 1 M glycine, pH 2.0 to remove secondary antisera then, reprobed with anti-phosphoserine antibodies (Chemicon; 1:1000 dilution). Lane 1: No TPA, lane 2: TPA added.