Table 2 of
Kyveris, Mol Vis 2002;
8:51-58.Table 2 of
Kyveris, Mol Vis 2002;
8:51-58.
Table 2. Comparison of total RNA derived from a variety of human ocular tissues using two different isolation methods
Average Average Average Average
Number of Ratio Sample RNA Yield Total RNA Yield
Tissue Method Samples (A260/A280) (mg) (mg) (mg/mg)
------- --------------------------- --------- ----------- ------- --------- ---------------
Iris Tri-Pure® Isolation Reagent 5 1.23 49 48 0.979
RNeasy® Midi Kit 4 1.87 111 14 0.126
Ciliary Tri-Pure® Isolation Reagent 5 1.38 505 54 0.107
body RNeasy® Midi Kit 4 1.95 237 23 0.097
Choroid Tri-Pure® Isolation Reagent 3 1.43 313 70 0.223
RNeasy® Midi Kit 4 1.90 459 93 0.203
Retina Tri-Pure® Isolation Reagent 5 1.40 602 50 0.083
RNeasy® Midi Kit 5 1.94 613 221 0.360
Optic Tri-Pure® Isolation Reagent 6 1.93 197 14 0.071
nerve RNeasy® Midi Kit 4 1.33 522 5 0.009
Sclera Tri-Pure® Isolation Reagent 7 1.84 306 14 0.046
RNeasy® Midi Kit 3 1.50 257 2.52 0.009
Cornea Tri-Pure® Isolation Reagent 9 1.83 175 19 0.108
RNeasy® Midi Kit 7 N/A 352 0 0
|