Figure 1 of
Vittitow, Mol Vis 2002;
Figure 1. Schematic representation of the construction of AdhRhoA2
AdhRhoA2 was generated by overlap recombination of a shuttle vector containing the full-coding mutated RhoA cDNA (pRG1) and a viral backbone derived from the parent vector Ad.Pacbgal upon restriction with Xba I/ ClaI. The shuttle plasmid was linearized with Age I and co-transfected with the Ad.Pacbgal arm into 293 cells by calcium phosphate/DNA co-precipitation. Recombinant AdhRhoA2 was obtained by plaque purification on 293 cells as described in Methods.