Table 2 of
Rengarajan, Mol Vis 2002;
8:416-421.Table 2 of
Rengarajan, Mol Vis 2002;
8:416-421.
Table 2. Comparison of the regression models of the different fluorophores
The models of ethidium bromide and Hoechst 33258 with higher concentrations of DNA were significant and had large R2 values indicating that they are appropriate for measuring DNA in concentrations of 0-20 μg/ml. The same flurophores were unsuitable for measuring lower concentrations of DNA (0-2 ng/ml). The models for PicoGreen and SYBR Green I also suggested that they are appropriate for measuring DNA in concentrations of 0-20 μg/ml. R-sq (R2) is the fraction of variability explained by the model, RMSE is the root mean square error (in counts/s), β1 (β1) is the estimated regression coefficient for the fluorescence intensity, and θ is the relative resolution. The values of θ may be compared between experiments of identical design.
A: High concentration of DNA (0-20 μg/ml)
Flurophore p value R-sq RMSE β1 θ ---------------- ------- ------ ------- ------- ----- Ethidium Bromide 0.0001 0.9937 35.48 0.06332 560.4 Hoechst 33258 0.0001 0.9925 1789. 5.940 301.2 |
B: Low concentration of DNA (0-2 ng/ml)
Flurophore p value R-sq RMSE β1 θ ---------------- ------- ------ ------- -------- ------ Ethidium Bromide 0.3000 0.0153 2.608 0.4776 5.462 Hoechst 33258 0.0998 0.0382 557.5 163.0 3.419 PicoGreen 0.0001 0.9737 8.032 71.66 0.1121 SYBR Green I 0.0001 0.9623 13.47 99.86 0.1349 |