Figure 19. Golgi-green protein expression in 293 cells

293 cells were transfected with the Golgi-pEGFP-N1 plasmid and observed for fluorescence 48 h later, using Epi-fl microscopy (EGFP filter set). A and B are two views of Golgi-green fluorescence. A was taken using the 40x oil objective and B was taken using the 60x oil objective. The images were obtained using a Nikon Optiphot-2 upright microscope with the episcopic-fluorescence attachment EFD-3. The cells observed in the images were photographed using a Nikon Coolpix 995 digital camera.