Figure 16. MP19^To3red protein expression in 293 cells

MP19^To3redTO cells were induced with tetracycline when the cells reached the correct cell density. About 7-10 days days following transfection, the cells were observed using Epi-fl microscopy (DsRed filter set). This is an Epi-fl image taken with the visible light lamp also turned on, revealing the overall cell shape as well as the red fluorescence of the MP19^To3 protein. A through C were taken with the focus at the top of the cell (A) and then manually traversing through the cell in three total steps to finally focus on the MP19^To3 protein (B through C). The images were obtained using a Nikon Optiphot-2 upright microscope with the episcopic-fluorescence attachment EFD-3. Cells were observed using a 60x oil objective. The cells observed in all of the images were photographed using a Nikon Coolpix 995 digital camera.