Figure 14. Observation of MP19^To3green protein in 293 cells

MP19^To3greenTO cells were induced with tetracycline when the cells reached the correct cell density. About 7-10 days days following transfection, the cells were observed using Epi-fl microscopy (EGFP filter set). A: MP19^To3greenTO fluorescent cells. The image were obtained using a Nikon Optiphot-2 upright microscope with the episcopic-fluorescence attachment EFD-3. Cells were observed using a 40x oil objective. In many cases, the fluorescence was so intense and bright that the image was very difficult to photograph. B: Conditions were identical with those in A, view of a different area of the coverslip. C: In many cases, after 7-10 days following induction of MP19^To3green protein expression, inclusion bodies of fluorescent protein was observed in the periphery of the cell (arrows). D: Conditions were identical with those in A, view of a different area of the coverslip, and the image photographed with the 60x oil objective. E: Magnified view of the inclusion body observed in C, lower arrow. The cells observed in all of the images were photographed using a Nikon Coolpix 995 digital camera.