Figure 11 of Chen, Mol Vis 2002; 8:372-388.


Figure 11. Mem-DsRed versus MP19green or MP19To3green protein expression in 293 cells

MP19greenTO (A and B) or MP19To3greenTO (C and D) cell lines were separately seeded onto coverslips and cotransfected with Mem-pDsRed2, a marker for the cell membrane fused to the red fluorescent protein, DsRed. After 48 h following transfection with pDsRed2-N1, the cells were induced with tetracycline and observed for fluorescence 24 h later. A: Analysis of MP19green and Mem-DsRed coexpression in 293 cells, using confocal microscopy with both the EGFP and DsRed filter sets. The image was obtained using the inverted microscope with a 40x water objective. B: Conditions were identical with those in A, view of a different area of the coverslip and the image was magnified 3-fold. C: Analysis of MP19To3green and Mem-DsRed coexpression in 293 cells, using confocal microscopy with both the EGFP and DsRed filter sets. The image was obtained using the inverted microscope with a 40x water objective. D: Analysis of MP19To3green and Mem-DsRed coexpression in 293 cells, using Epi-fl microscopy (EGFP and DsRed filter sets). The image was obtained using a Nikon Optiphot-2 upright microscope with the episcopic-fluorescence attachment EFD-3. Cells were observed using a 60x oil objective.

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Chen, Mol Vis 2002; 8:372-388 <http://www.molvis.org/molvis/v8/a45/>
©2002 Molecular Vision <http://www.molvis.org/molvis/>
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