Figure 10 of
Chen, Mol Vis 2002;
Figure 10. DsRed versus MP19green or MP19To3green protein expression in 293 cells
MP19greenTO (A) or MP19To3greenTO (B) cell lines were separately seeded onto coverslips and cotransfected with the empty pDsRed2-N1. This vector expressed DsRed as a cytoplasmic, soluble protein in the cells. After 48 h following transfection with pDsRed2-N1, the cells were induced with tetracycline and observed for fluorescence 24 h later, using confocal microscopy with both the EGFP and DsRed filter sets. The image was obtained using the inverted microscope with a 40x water objective.