Figure 1 of Acharya, Mol Vis 2002; 8:367-371.

Figure 1. Detection of the p53 polymorphism and construction of haplotype

A: Locations of the polymorphic sites within the p53 gene and the nature of the polymorphisms are shown. The allelic difference resulting from codon 72 polymorphism were determined by BstU I digestion of the PCR product obtained by using a pair of primers selected from exon 3 and intron 4 of the gene. B: All possible combinations of DNA fragments resulting from BstU I digestion of the PCR products and the haplotypes determined based on the digestion pattern are shown. C: Polyacrylamide gel (6%) analysis of PCR products from five representative samples, with (+) and without (-) BstU I digestion, is shown. The haplotypes determined based on the analysis are indicated at the bottom of the panel. The ins-Arg haplotype has not been detected in any sample. Lane M is a pBS/Hae III digested molecular weight marker. The sizes of the molecular weight marker and the BstU I digest are shown in the left and the right sides of the gel, respectively. DNA bands present only in samples 4 and 5 (four right lanes) above 458 bp size marker are due to heteroduplex formation between the 448 bp and 432 bp bands, which are retained in the native polyacrylamide gel.

(70 K)

Acharya, Mol Vis 2002; 8:367-371 <>
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