Table 2 of
Anderson, Mol Vis 2002;
8:351-358.
Table 2. Fatty acid composition of phospholipids from P23H mutant rat ROS
Lipids were extracted from ROS prepared from retinas of 21-22 day-old rats. Total phospholipids were prepared by thin layer chromatography and converted to methyl esters, which were analyzed by gas-liquid chromatography. The values are relative mole percent ± standard deviation.
Line 2 Line 3 Wild-Type (slow) (intermediate) Line 1 (fast) ------------ ------------ ---------------- -------------------- Avg SD Avg SD Avg SD * Avg SD * Fatty Acid (n=6) (n=6) (n=5) (n=5) ---------- ----- ---- ----- ---- ----- ---- - ----- ---- ----- Saturated 14:0 0.72 0.18 0.73 0.17 0.74 0.09 0.92 0.15 16:0 19.68 1.99 21.56 1.46 21.91 0.93 a 23.66 0.50 c,e,j 18:0 22.05 0.90 22.21 0.72 23.32 1.48 23.94 0.66 c,f 20:0 0.17 0.07 0.13 0.03 0.14 0.18 0.27 0.17 22:0 0.03 0.00 0.05 0.01 0.07 0.02 0.14 0.11 24:0 0.07 0.08 0.04 0.03 0.09 0.06 0.08 0.03 Subtotal 42.73 2.34 44.72 1.74 46.28 2.03 a 49.00 1.02 c,g,h Monoenoic 16:1 0.46 0.19 0.56 0.09 0.65 0.25 1.07 0.41 a,e 18:1 8.84 1.91 10.68 1.51 10.93 0.84 a 13.23 0.18 c,f,k 20:1 0.18 0.04 0.21 0.03 0.23 0.11 0.27 0.08 22:1 0.36 0.39 0.24 0.11 0.39 0.15 0.56 0.61 24:1 0.07 0.05 0.07 0.05 0.11 0.04 0.10 0.07 Subtotal 9.91 2.43 11.76 1.64 12.31 0.89 15.23 0.60 c,g,k N-6 18:2 1.07 0.79 1.12 0.51 0.93 0.43 0.97 0.66 18:3 0.08 0.01 0.07 0.02 0.06 0.01 0.05 0.02 20:3 0.26 0.02 0.23 0.01 0.24 0.02 0.20 0.03 20:4 7.05 0.59 7.33 0.36 7.76 0.37 a 8.61 0.47 c,g,j 22:4 1.41 0.09 1.52 0.07 1.63 0.07 c 1.85 0.20 b,e 22:5 0.54 0.04 0.55 0.03 0.54 0.08 0.50 0.05 24:4 0.39 0.07 0.27 0.06 0.26 0.10 0.16 0.03 c,f 24:5 0.08 0.02 0.08 0.01 0.09 0.02 0.12 0.03 Subtotal 10.88 0.57 11.18 0.42 11.51 0.30 a 12.46 0.54 d,g,j N-3 18:3 0.13 0.05 0.13 0.04 0.15 0.08 0.17 0.06 20:5 0.11 0.01 0.09 0.04 0.14 0.01 0.15 0.03 22:5 0.53 0.02 0.53 0.04 0.57 0.02 a 0.61 0.04 c,f 22:6 34.77 3.67 30.80 3.39 28.45 1.58 b 21.91 0.85 d,g,k 24:5 0.28 0.12 0.24 0.05 0.18 0.07 0.17 0.08 24:6 0.66 0.09 0.55 0.11 0.42 0.12 b 0.30 0.17 e Subtotal 36.48 3.89 32.34 3.51 29.90 1.65 b 23.31 1.06 d,g,k N-6/N-3 0.30 0.04 0.35 0.05 0.39 0.03 c 0.54 0.04 d,g,k PL/Prot 0.99 0.22 1.03 0.20 1.25 0.26 1.13 0.26 *Notes: a: p<0.05 compared to wild type b: p<0.01 compared to wild type c: p<0.005 compared to wild type d: p<0.001 compared to wild type e: p<0.05 compared to line 2 (slow) f: p<0.01 compared to line 2 (slow) g: p<0.005 compared to line 2 (slow) h: p<0.05 compared to line 3 (intermediate) j: p<0.01 compared to line 9 (intermediate) k: p<0.005 compared to line 9 (intermediate) |