Table 1 of
Anderson, Mol Vis 2002;
8:351-358.Table 1 of
Anderson, Mol Vis 2002;
8:351-358.
Table 1. Fatty acid composition of phospholipids from S334ter mutant rat ROS
Lipids were extracted from ROS prepared from retinas of 21-22 day-old rats. Total phospholipids were prepared by thin layer chromatography and converted to methyl esters, which were analyzed by gas-liquid chromatography. The values are relative mole percent ± standard deviation.
Wild-Type Line 9 (slow) Line 4 (intermediate)
------------ ---------------- ---------------------
Avg SD Avg SD * Avg SD *
Fatty Acid (n=6) (n=9) (n=6)
--------------- ----- ---- ----- ---- - ----- ---- ---
Saturated
14:0 0.55 0.11 0.65 0.24 0.73 0.15 a
16:0 17.65 2.31 19.11 1.24 21.51 1.48 c,f
18:0 22.66 1.05 22.38 1.15 23.45 0.78 e
20:0 0.16 0.08 0.10 0.11 0.25 0.06
22:0 0.05 0.02 0.06 0.03 0.06 0.02
24:0 0.04 0.01 0.09 0.08 0.06 0.03
Subtotal 41.12 3.40 42.39 1.01 46.06 1.63 a,g
Monoenoic
16:1 0.54 0.09 0.61 0.15 0.66 0.15
18:1 7.45 1.41 9.54 1.81 a 10.85 1.77 c
20:1 0.16 0.04 0.18 0.05 0.22 0.06
22:1 0.34 0.28 0.82 0.93 0.48 0.28
24:1 0.08 0.08 0.08 0.08 0.29 0.57
Subtotal 8.57 1.52 11.22 1.84 b 12.50 2.35 b
N-6
18:2 0.75 0.12 1.18 0.79 0.81 0.31
18:3 0.08 0.02 0.08 0.03 0.04 0.02
20:3 0.24 0.01 0.26 0.03 0.24 0.02
20:4 6.61 0.37 7.20 0.55 a 7.49 0.26 c
22:4 1.32 0.14 1.48 0.12 a 1.65 0.13 c,e
22:5 0.54 0.09 0.49 0.05 0.54 0.04
24:4 0.46 0.09 0.39 0.05 0.27 0.04 c,g
24:5 0.06 0.02 0.07 0.02 0.08 0.02
Subtotal 10.05 0.60 11.15 0.62 b 11.13 0.45 b
N-3
18:3 0.13 0.03 0.15 0.05 0.17 0.07
20:5 0.10 0.02 0.11 0.02 0.10 0.02
22:5 0.49 0.06 0.52 0.04 0.56 0.05 a
22:6 38.42 4.93 33.57 2.68 a 28.69 3.99 c,e
24:5 0.35 0.07 0.28 0.04 0.23 0.05 b
24:6 0.76 0.17 0.61 0.06 0.56 0.15
Subtotal 40.26 5.13 35.23 2.73 30.31 4.07 c,e
N-6/N-3 0.25 0.04 0.32 0.04 a 0.37 0.06 c
PL/Prot (wt/wt) 1.09 0.24 1.15 0.24 1.20 0.40
*Notes:
a: p<0.05 compared to wild type
b: p<0.01 compared to wild type
c: p<0.005 compared to wild type
e: p<0.05 compared to line 9 (slow)
f: p<0.01 compared to line 9 (slow)
g: p<0.005 compared to line 9 (slow)
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