Figure 6. PARP cleavage correlates with the presence of functional alleles of p53

Immunohistochemical staining for cleaved PARP in (A) p53+/+ mice, and (B) p53-/- mice, 2 days after intravitreal injection of 160 nmol of NMDA. Positive staining of the nuclei and inner plexiform layer is restricted to mice carrying functional p53, while none of the p53-/- mice examined had any detectable staining. Positive staining for cleaved PARP was also present in p53+/- mice (data not shown), although it was less intense and variable through out the ganglion cell layer. Size bar represents 15 μm.