Figure 2 of
Li, Mol Vis 2002;
Figure 2. Upregulation of p53 expression coincides with early cell death
The upregulation of p53 expression coincides with the period of early cell death. Top Panel: p53 message levels were measured by RT-PCR analysis followed by Southern blot analysis using a murine p53 probe. Shown is an autoradiograph of a representative blot. Lower Panel: Actin message levels in the same samples were measured by RT-PCR and the products detected by staining with ethidium bromide. Shown is a negative image of a representative ethidium bromide-stained gel. The level of p53 was quantified as described in the Methods. Elevated levels of the p53 cDNA fragment are consistently observed in the retinas 3 h after NMDA injections.