Table 1 of
Wordinger, Mol Vis 2002;
8:241-250.Table 1 of
Wordinger, Mol Vis 2002;
8:241-250.
Table 1. PCR primer pairs
Table 1 describes the expected sizes of PCR amplification products with each human BMP and BMP receptor primer pair and optimal annealing temperature. Primers were designed using Oligo 4.0 (National Biosciences, Plymouth, MN).
Accession Size Annealing
Gene Number Upstream Primer (5'-3') Downstream Primer (5'-3') (bp) Temp (°C)
------------- --------- -------------------------- ------------------------- ---- ---------
BMP-2 NM-001200 GGAAGAACTACCAGAAACGAG AGATGATCAGCCAGAGGAAAA 657 55
BMP-4 NM_001202 ACCTGAGACGGGGAAGAAAA TTAAAGAGGAAACGAAAAGCA 348 55
BMP-5 NM_021073 AAGAGGACAAGAAGGACTAAAAATAT GTAGAGATCCAGCATAAAGAGAGGT 303 55
BMP-7 NM_001719 AGCCCGGGTAGCGCGTAGAG GCGCCGGTGGATGAAGCTCGA 202 60
BMP-RIA NM_004329 TAAAGGTGACAGTACACAGGAACA TCTATGATGGCAAAGCAATGTCC 298 55
BMP-RIB NM_001203 TACAAGCCTGCCATAAGTGAAGAAGC ATCATCGTGAAACAATATCCGTCTG 211 55
BMP-RII NM_001204 TCCTCTCATCAGCCATTTGTCCTTC AGTTACTACACATTCTTCATAG 457 55
Cer-1 NM_005454 ATAGTGAGCCCTTCCCACCT AATGAACAGACCCGCATTTC 294 60
Chordin AF209930 CTCTGCTCACTCTGCACCTG CCGGTCACCATCAAAATAGC 198 60
DRM (Gremlin) NM_013372 ATCAACCGCTTCTGTTACGG ATGCAACGACACTGCTTCAC 197 60
Follistatin NM_006350 TGCCACCTGAGAAAGGCTAC ACAGACAGGCTCATCCGACT 201 60
Noggin NM_005450 CACTACGACCCAGGCTTCAT CTCCGCAGCTTCTTGCTTAG 212 60
NMA (BAMBI) XM_005791 GATCGCCACTCCAGCTACATC GGGCACGGCAATGACC 471 58
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