Figure 3. Detection of apoptotic and antiapoptotic markers by RT-PCR

Total cellular RNA was isolated from lens epithelial cells of patients with non-cataractous clear lenses (lane 1), nuclear cataracts (lane 2), and anterior polar cataracts (lane 3). The levels of mRNAs for Bax, Bcl-2, and GAPDH were examined by RT-PCR. The data shown are from one of three independent assays that produced similar results.