Figure 2. DNA fragmentation assay for human anterior polar cataracts

Total genomic DNA was isolated from lens epithelial cells of patients with nuclear cataracts (lane 1), anterior polar cataracts (lane 2), and non-cataractous clear lenses (lane 3). The three DNA samples were separated in 2.0% agarose gel, stained with ethidium bromide, and photographed under UV illumination. Each genomic DNA was isolated and pooled from the portions of the same lens capsule epithelial cells used for TUNEL assay. Lane M was the 100 bp ladder molecular size marker.