Figure 1 of
Lee, Mol Vis 2002;
Figure 1. Effect of FGF-2 on PLC enzyme activity
CEC at 60% confluency were labeled with myo-(2-3H) inositol in the inositol- and serum-free DMEM for 24 h. Cells were then treated either with FGF-2 (10 ng/ml) for up to 48 h (A) or with FGF-2 in concentrations ranging from 0.01 ng/ml to 50 ng/ml for 1 h (B). Total inositol phosphate formation was measured as described in the text. FGF-2 treatment was accompanied with heparin 10 (mg/ml) as described in the text. Data are presented as x-fold stimulation when compared to the level of total inositol phosphates accumulation performed at time 0 (A) or in the absence of FGF-2 (B). Experiments were done in triplicate and repeated four times; data shown are from one experiment. Error bars represent the standard error of the mean.