Figure 4 of Wistow, Mol Vis 2002; 8:196-204.


Figure 4. Alternative splicing in PDE6G

The complete sequence of PDE6G deduced from hd/he retina ESTs is shown, colored according to exons in the human genome. Exon 1 is in red, exon 2 in blue, exon 3 in purple, and exon 4 in black. Sequence in lower case is noncoding, upper case is coding. Protein sequence is shown above the DNA sequence. In three ESTs, exon 2 is entirely skipped. The resulting shortened ORF is indicated by the green and brown protein sequence and the start sites of the canonical (1) and short (2) ORFs are indicated with arrows. The brown protein sequence shows the three α-helices involved in α-transducin binding. The polyadenylation site is shown in turquoise.

ggccggggctggcaccctgcggagggaggcccagcactcacagcacagcc   50

ccctgagacccgccctgcacttgaccgcagcaggagggagtccaggagcc  100
                                 ->1
                                 M  N  L  E  P  P        6
aaggttgccgcggtgtctccgtcagcctcaccATGAACCTGGAACCGCCC  150
 K  A  E  F  R  S  A  T  R  V  A  G  G  P  V  T  P      23
AAGGCTGAGTTCCGGTCAGCCACCAGGGTGGCCGGGGGACCTGTCACCCC  200
  R  K  G  P  P  K  F  K  Q  R  Q  T  R  Q  F  K        39
CAGGAAAGGGCCCCCTAAATTTAAGCAGCGACAGACCAGGCAGTTCAAGA  250
S  K  P  P  K  K  G  V  Q  G  F  G  D  D  I  P  G       56
GCAAGCCCCCAAAGAAAGGCGTTCAAGGGTTTGGGGACGACATCCCTGGA  300
 ->2
 M  E  G  L  G  T  D  I  T  V  I  C  P  W  E  A  F      73
ATGGAAGGCCTGGGAACAGACATCACAGTCATCTGCCCTTGGGAGGCCTT  350
  N  H  L  E  L  H  E  L  A  Q  Y  G  I  I  *           87
CAACCACCTGGAGCTGCACGAGCTGGCCCAATATGGCATCATCTAGcacg  400
aggccctgctgaagtccagaccctccccctcctgcccactatgctctaaa  450
ccctgctcaggattcctgttgaggagatgcctccctagcccagatggcac  500
ctggacaccaggatgggactgcaacctcaggtctccccctacatattaat  550
accagtcaccaggagcccaccacctccctctaggatgccccctcaggggc  600
tggccaggccctgctcaacatctggagacacaggcccacccctcagtcct  650
gcccacagagaggcttggtcggtctccactcccagggagaacgggaagtg  700
gaccccagcccgggagcctgctggaccccagatcgtcccctcctcccagc  750
tggaaagctagggcaggtctccccagagtgcttctgcaccccagccccct  800
gtcctgcctgtaaggggatacagagaagctccccgtctctgcatcccttc  850
ccaggggggtgcccttagtttggacatgctgggtagcaggactccagggc  900
gtgcacggtgagcagatgaggccccaagctcatcacaccagggggccatc  950
cttctcaatacagcccgcccttgcagtccctatttcaaaataaaattagt 1000
gtgtccttg(polyA)                                   1009

Wistow, Mol Vis 2002; 8:196-204 <http://www.molvis.org/molvis/v8/a26/>
©2002 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535