Figure 1 of
Wistow, Mol Vis 2002;
8:196-204.Figure 1 of
Wistow, Mol Vis 2002;
8:196-204.
Figure 1. Sequence and gene structure of human retbindin
A: The cDNA sequence of human retinal retbindin (GenBank accession number AY028917) is shown in red. The alternative first exon (partial) is shown in blue. The sequence is mapped onto human genome sequence to show exon/intron structure. Nucleotide numbers are from BAC clone AC020934. The predicted protein sequence is shown in single letter code. The polyadenylation site is in green and a potential TATA box is capitalized. B: A diagram of retbindin gene organization showing the pattern of alternative splicing. Coding sequence (ORF) is indicated in green with UTRs in orange. Two alternative first exons are numbered 1a and 1b. Intron sizes are shown.
A
cacaaggaTATATAcacaccagccccaagtcggccagcctttggtcagctggagtgtggg
caggacggtgggaggtcccgcccctgcattaagccacgcccctttcagcagccagtttcc
tggactggatcctgaggtgtcttaGCGGCCTCTAGACCCCAATCCAGCCAACAGgtgagg Ex1a
--- 180 bp ---
gaagggccggcagggagcgagagagggaggagGGAGGCCGCATAGAAGAGAGGGAGGAAT Ex1b
TACTGAACAGGAGAGGGGGATTGGAAGACCTAGGGAGGTCCCCTTCATTCTGAGAAAAGA
AGGGGTCTCTTGAAGACTAAAAAGgtgggagatcctgctggatgggaggagggccactgt
--- 180 bp ---
atgcactgggtgggtgccaccctaagctcacagaaagggatggctgaaccccaccctatg
M D C R V H M R P I G 11
cctgccagCTCTTATCTCAGGTGGACATGGACTGCAGGGTCCACATGCGACCCATCGGCC Ex2
L T W V L Q L T L A W I L L E A C G G S 31
TGACGTGGGTGCTGCAACTGACCTTGGCATGGATCCTGCTAGAAGCCTGTGGAGGGAGCC
R P L Q A R S Q Q H H G L A A D L G K G 51
GCCCACTCCAAGCCAGGTCCCAGCAACACCATGGGCTGGCAGCTGATCTGGGCAAAGGCA
K L H L A 56
AGCTGCACCTGGCAGgtgagcaccccagaccctgcctcaccaccaacacccagaaaccca
--- 780 bp ---
G P C C P S E M D T 66
agaccctagctcacagggttcccctttgccagGACCTTGTTGTCCCTCAGAGATGGACAC Ex3
T E T S G P G N H P E R C G V P S P E 85
AACAGAGACATCGGGCCCTGGAAACCATCCAGAACGCTGTGGAGTGCCGAGCCCTGAgtg
agtacagcaccctccaccgttttctcgcgtactttgcccattgcccaggaactccctcgg
C E S F L E H 92
gtcctgacccatccgaccctatctctcccaacgtccagATGCGAATCCTTCCTGGAACAC Ex4
L Q R A L R S R F R L R L L G V R Q A Q 112
CTCCAACGTGCCCTTCGCAGTCGCTTCCGCCTGCGGCTATTGGGGGTACGCCAGGCACAG
P L C E E L C Q A W 122
CCGCTCTGCGAGGAGCTCTGCCAGGCCTGgtaggagacgcgggggcaaggggcgtgggtt
--- 1740 bp ---
F A N C E D D I T C G P T W L 137
ctcgccatctccagGTTCGCCAACTGCGAAGATGATATCACCTGCGGCCCGACTTGGCTC Ex5
P L S E K R G C E P S C L T Y G Q 154
CCACTCTCAGAAAAAAGGGGCTGTGAGCCCAGCTGCCTTACCTATGGACAGgtgagcgtg
gggcagaaggtgaagggaagagactgagatcctccccaccaagttatttatttatttatt
--- 720 bp ---
T F A 157
ccccacgtctctggactccgcccactagggccttaactccactttttcctagACCTTCGC Ex6
D G T D L C R S A L G H A L P V A A P G 177
AGACGGGACGGACCTTTGTCGCTCGGCTCTGGGCCACGCCCTACCGGTGGCTGCTCCTGG
A R H C F N I S I S A V P R P R P G R R 197
AGCCCGTCACTGCTTCAACATCTCCATCTCCGCGGTACCTCGTCCCAGACCAGGACGACG
G R E A P S R R S R S P R T S I L D A A 217
GGGCCGGGAAGCTCCCTCCCGGCGTTCCCGCAGCCCTCGCACCTCCATCCTGGACGCTGC
G S G S G S G S G S G P * 229
GGGCAGCGGGAGTGGCAGTGGAAGCGGCAGCGGCCCCTAGCGGACGCGTGGCCCTGAGTT
GGGGGAGCGACCCTTCCCCCAGCCCCGCCCCTCAGGACACCCAGAACCCCACCCCTCGTC
CTCTCGGCCTTCTGTAATAGTTTTGAGATGTCTGTCCCTCCTCCCTGGAGCTCCAGAGAC
CCACCCCTCTCCAGGTTATCCCAGAAATGACCCAACTCTCTCACTTTTCCCTCTCCCCTT
TGAATAAAGTCGCCAGCTAGAGCacgtgacgagtgtggctgcttcttggggaaataagtc
|
B
