Table 2 of
Garcia, Mol Vis 2002;
8:10-16.Table 2 of
Garcia, Mol Vis 2002;
8:10-16.
Table 2. Lectin staining in cryosections
This table summarizes the fungal staining obtained with different lectins at several concentrations on frozen tissue. "++" denotes bright staining; "+" denotes moderate staining; "+/-" denotes uneven staining; "-" denotes background staining. We found important differences in fungal and corneal staining with different lectin concentrations. The identification of the microorganisms was easier in frozen sections than in paraffin specimens (compare with Table 3).
Concentration
Lectins (mg/ml) C. albicans A. fumigatus F. solani
------- ------------- ----------- ------------ ---------
Con A 1 - - -
5 - - -
10 + + +/-
50 + +/- +/-
100 - - -
WGA 1 - + +
5 + ++ ++
10 + ++ ++
50 +/- +/- +/-
100 - + -
500 - - -
PKM 10 - - -
50 - +/- +/-
100 +/- +/- +/-
500 - +/- +/-
1000 - +/- -
GNA 1 +/- +/- -
5 +/- +/- +/-
10 +/- +/- +/-
50 - - -
100 - - -
SWGA 1 - ++ +
5 + + +
10 - +/- +
50 - - -
100 - - -
500 - - -
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