Figure 3. Predicted misfolding of first generation anti-V230 hRz

In the misfolded conformer (II) the 3' AS flank folds anomalously into the StemII/loop domain obviating the formation of an enzymatic structure. The AS flanks are shown in black and the core consensus enzyme in bold blue. The free energies of the misfolded and desired hRz conformations are shown. Thermodynamic equations describing the relationship between DG and the equilibrium constant are shown for each conformation. The ratio of the equilibrium constants indicates that the desired enzymatically active conformation is a small fraction of all hRz RNA.