Figure 4 of
Wang, Mol Vis 2002;
Figure 4. Nuclear extracts from corneal epithelial cells bind regions shown in footprinting assays
The synthetic double-stranded DNA probes corresponding to regions of -111 to -182 bp and -193 to -256 bp upstream of the Krt1.12 gene were labeled with [γ-32P]dCTP and used in EMSAs. Nuclear extracts were prepared from bovine corneal epithelial cells. In both regions, the bindings of corneal epithelial nuclear extracts were specific and could be competed with unlabeled probe. However, the probes also bound nuclear extracts from retinal tissue. Nuclear extract is designated ND and retina is designated R.