Figure 2. In situ histochemical staining of β-gal in cornea and conjunctiva

Keratin 12 promoter-β-gal constructs were coated onto 0.6 μm gold particles and delivered to cornea/conjunctival junctions at 200 psi. The tissues were collected at 48 h and subjected to staining with X-gal as described in Methods. PNASSβ (C) generated very few positive reactions in both cornea and conjunctiva. The 0.2 kb construct (A) generated positive reactions in both cornea and conjunctiva. The expression of β-gal by the 0.4 kb construct of the 5' flanking sequence of the Krt1.12 gene is restricted to the cornea (B). The arrows indicate the boundary between cornea and conjunctiva.