Figure 1. In vivo β-gal expression by Krt1.12 promoter-β-gal constructs

Gold particles (0.6 μm) coated with Krt1.12 constructs were bombarded onto rabbit corneas at 150 psi. Another set of gold particles (1.6 mm) coated with Krt1.12 constructs were bombarded onto rabbit bulbar conjunctivas at 150 psi and onto rabbit dorsal skin at 400 psi (8 mm in diameter). The tissues were collected 48 h after delivery. The enzyme activities of β-gal were determined as describe in Methods. Values are averages of β-gal activities ±standard deviations of 8 specimens from individual tissues.