Figure 7 of
Ostrovsky, Mol Vis 2002;
8:72-78.
Figure 7. SDS-PAGE and western blot analysis of rbA3-crystallin
SDS-PAGE (A) and western blot (B) obtained for UV irradiated rbA3-crystallin on apparatus B. Lane 1 is control rbA3; lane 2 is non-irradiated rbA3; lanes 2,3 and 4 are soluble fractions of rbA3 irradiated at 308 nm with doses 26 J/cm2, 50 J/cm2 and 170 J/cm2, respectively; lanes 6 and 11 show protein standards; lanes 7, 8 and 9 are insoluble fractions of rbA3 irradiated at 308 nm with 26 J/cm2, 50 J/cm2, 170 J/cm2, respectively, and dissolved in 4% SDS; lane 10 is insoluble fraction of non-irradiated rbA3 dissolved in 4%SDS.