Figure 6 of
Ostrovsky, Mol Vis 2002;
8:72-78.
Figure 6. Chromatographic profiles of rbA3-crystallin
Chromatographic profiles correspond to the non-irradiated and UV irradiated at doses 26 J/cm2, 50 J/cm2 and 170 J/cm2 rbA3-crystallin, respectively, on apparatus B. Samples of 0.1 ml were loaded to the Superdex 75 10/30HR column at 0.5 ml/min. Protein standards used in gel-filtration chromatography from left to right are bovine serum albumin, ovalbumin, carbonic anhydrase, chymotrypsinogen A, cytochrome c, ribonuclease A and aprotinin, respectively. Void and column volumes are 7.9 ml and 24 ml, respectively.