Figure 4. Subcellular localization of P4Ha with PDI and Grp78/Bip

CEC cells were fixed, permeabilized, and stained with antibodies as described in the methods. Some cells were treated with 0.3 mM a,a'-dipyridyl for 2 h prior to fixing and staining (C and D). A and C: Cells were stained for Grp78 (green) and P4Ha (red). B and D: Cells were stained for PDI (green) and P4Ha (red). Bar represents 10 mm. The data represent four independent experiments.