Figure 2. Subcellular localization of procollagen I and type IV collagen with P4Ha

CEC cells were fixed, permeabilized, and stained with antibodies as described in the methods. Some cells were treated with 0.3 mM a,a'-dipyridyl for 2 h prior to fixation and staining (C and D). A and C: Cells were stained for procollagen I (red) and P4Ha (green). B and D: Cells were stained for type IV collagen (green) and P4Ha (red). Bar represents 10 mm. The data represent four independent experiments.