Figure 4 of
Chen, Mol Vis 2001;
Figure 4. Localization of GRK7 in human retina
A and B: Chromogenic detection of hGRK7 by UU45APC (1:1,000 dilution, A) and pre-absorbed UU45APC (1:500 dilution, B) on sections of a 2 h fixed retina from an adult donor 3 h postmortem. Bar represents 20 mm. C to E: Fluorescent detection of GRK7 in sections of an overnight fixed adult retina >6 h postmortem. C: Labeling of cone sheath by rhodamine conjugated peanut agglutinin. D: Labeling of the photoreceptor layer using UU45APC (1:1,000 dilution) visualized by FITC conjugated secondary antibody. E: Co-labeling of UU45APC and peanut agglutinin. F to H: Fluorescent staining of a 1 h fixed retina from a 4 month old female whose eye was enucleated due to retinoblastoma. The eye was received 2 h post enucleation. F: Labeling of cones using monoclonal antibody 7G6 (1:100 dilution) visualized by rhodamine conjugated secondary antibody. G: Labeling of the photoreceptor layer using UU45APC (1:500 dilution) visualized by FITC conjugated secondary antibody. H: Co-labeling of UU45APC and 7G6. Bar represents 10 mm. GRK7 is present in most retinal layers, but is absent in rod outer segments (A). The presence of GRK7 in the cone outer segments is obvious in D, E, G, and H. However, the proximal part of cone outer segments is more intensively labeled by UU45APC in the 4 month old retina fixed for 1 h. OS: outer segments, IS: inner segments, ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, GCL: ganglion cell layer.