Figure 4. Effect of TGF-b2 on Cdk4 and p27 in actively cycling CEC

Rabbit CEC were treated with 10 ng/ml TGF for 24 h in DMEM-10. Cells were then fixed and stained. Cdk4 was stained with rhodamine-conjugated secondary antibody, and p27 was stained with FITC-conjugated secondary antibody. A and B are primary CEC, C and D are first passage CEC, and E and F are second passage CEC. Data are from three experiments. Primary cultures are labelled "Pr", first passage cultures are labelled "1°", and second passage cultures are labelled "2°". Bar equals 20 mm.