Figure 1. Cdk4 and p27 in first passage rabbit CEC

Actively growing cells (for Cdk4) or cells reaching confluency (for p27) were treated either with FGF-2 (10 ng/ml) supplemented with heparin (10 mg/ml) in the absence of serum or with TGF-b2 (10 ng/ml) in DMEM-10 for 24 h. Cells were washed, fixed in 4% paraformaldehyde and stained as described in the text. Control experiments (A,B) were performed in parallel with the omission of the primary antibodies. The panels labeled D0 (C,D) are cells maintained in serum free DMEM for 24 h. Data are from two experiments. Primary cultures are labelled "Pr". Bar equals 20 mm.