Figure 7. Effect of pseudosubstrates on PKC activity

Effect of PKC-g and PKC-a pseudosubstrate peptides on total PKC activity in lens cortical fiber cells. Cortical fiber cells were isolated and homogenized, then they were incubated with different concentrations of PKC-g or PKC-a pseudosubstrate peptides for 1 h. PKC activity was then determined using a PKC assay kit. In the control experiments, no pseudosubstrate was added. The concentrations of the pseudosubstrate peptide that were added to the cells were: 10 nM, 100 nM, and 1 mM.