Figure 6. Threonine phosphorylation on Cx46

Inhibition of threonine phosphorylation on Cx46 by myristoylated pseudosubstrate inhibitory peptide against PKC-g in the lens cortex. Fiber cells of the lens cortex were dissected and incubated with different concentrations of myristoylated pseudosubstrate peptide specific for PKC-g, then Cx46 was immunoprecipitated and the samples were analyzed by SDS-PAGE and western blot. The transferred proteins on western blots were probed with anti-phosphothreonine antibodies. A: Western blots showing phosphorylated threonine on Cx46 after treatment with different concentrations of PKC-g pseudosubstrate inhibitory peptide (lane 1: Control, no pseudosubstrate peptide; lane 2: 10 nM peptide; lane 3: 100 nM peptide; lane 4: 1 mM peptide). B: Quantitative analysis of inhibition of threonine phosphorylation on Cx46 in the lens cortex by PKC-g pseudosubstrate inhibitory peptide.