Figure 1 of
Demirci, Mol Vis 2001;
7:234-239.Figure 1 of
Demirci, Mol Vis 2001;
7:234-239.
Figure 1. APT6M8-9 cDNA sequence together with translation and exon-intron boundaries locations
The color scheme is as follows: black, HSM800272 partial cDNA sequence published in GenBank; blue, 5' end cDNA sequence identified by standard RACE; red, 5' end cDNA sequence identified by RLM-RACE; orange, nucleotides flanking an intron (exon-intron junction). The nucleotide at position 1012 was found to be an "A" in 8 people we have screened, the same as in the NM_005765 sequence from GenBank. However, the GenBank cDNA clones HSM800272 and AF248966 indicate a "G" at this location which encodes Arginine instead of Glutamine. Similarly, we found an "A" at position 1476 (3' UTR) which is indicated as a "G" in GenBank HSM800272 and AF248966 sequences.
10 20 30 40 50 60 70 80 90 100
GTGGAGAAAGCGGCAGCTGTCCCAGCGGAAGCGACGAAGGGACGGGACCCGGGAGCCTGGACGAGTCCGAGCGCGTCACCTCCTCACGCTGCGGCTGTCG
110 120 130 140 150 160 170 180 190 200
CCCGTGTCCCGCCGGCCCGTTCCGTGTCGCCCCGCAGTGCTGCGGCCGCCGCGGCACCATGGCTGTGTTTGTCGTGCTCCTGGCGTTGGTGGCGGGTGTT
M A V F V V L L A L V A G V
210 220 230 240 250 260 270 280 290 300
TTGGGGAACGAGTTTAGTATATTAAAATCACCAGGGTCTGTTGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGAGCGGATCCCAGACGTGGCTGCAT
L G N E F S I L K S P G S V V F R N G N W P I P G E R I P D V A A
310 320 330 340 350 360 370 380 390 400
TGTCCATGGGCTTCTCTGTGAAAGAAGACCTTTCTTGGCCAGGACTCGCAGTGGGTAACCTGTTTCATCGTCCTCGGGCTACCGTCATGGTGATGGTGAA
L S M G F S V K E D L S W P G L A V G N L F H R P R A T V M V M V K
410 420 430 440 450 460 470 480 490 500
GGGAGTGAACAAACTGGCTCTACCCCCAGGCAGTGTCATTTCGTACCCTTTGGAGAATGCAGTTCCTTTTAGTCTTGACAGTGTTGCAAATTCCATTCAC
G V N K L A L P P G S V I S Y P L E N A V P F S L D S V A N S I H
510 520 530 540 550 560 570 580 590 600
TCCTTATTTTCTGAGGAAACTCCTGTTGTTTTGCAGTTGGCTCCCAGTGAGGAAAGAGTGTATATGGTAGGGAAGGCAAACTCAGTGTTTGAAGACCTTT
S L F S E E T P V V L Q L A P S E E R V Y M V G K A N S V F E D L
610 620 630 640 650 660 670 680 690 700
CAGTCACCTTGCGCCAGCTCCGTAATCGCCTGTTTCAAGAAAACTCTGTTCTCAGTTCACTCCCCCTCAATTCTCTGAGTAGGAACAATGAAGTTGACCT
S V T L R Q L R N R L F Q E N S V L S S L P L N S L S R N N E V D L
710 720 730 740 750 760 770 780 790 800
GCTCTTTCTTTCTGAACTGCAAGTGCTACATGATATTTCAAGCTTGCTGTCTCGTCATAAGCATCTAGCCAAGGATCATTCTCCTGATTTATATTCACTG
L F L S E L Q V L H D I S S L L S R H K H L A K D H S P D L Y S L
810 820 830 840 850 860 870 880 890 900
GAGCTGGCAGGTTTGGATGAAATTGGGAAGCGTTATGGGGAAGACTCTGAACAATTCAGAGATGCTTCTAAGATCCTTGTTGACGCTCTGCAAAAGTTTG
E L A G L D E I G K R Y G E D S E Q F R D A S K I L V D A L Q K F
910 920 930 940 950 960 970 980 990 1000
CAGATGACATGTACAGTCTTTATGGTGGGAATGCAGTGGTAGAGTTAGTCACTGTCAAGTCATTTGACACCTCCCTCATTAGGAAGACAAGGACTATCCT
A D D M Y S L Y G G N A V V E L V T V K S F D T S L I R K T R T I L
1010 1020 1030 1040 1050 1060 1070 1080 1090 1100
TGAGGCAAAACAAGCGAAGAACCCAGCAAGTCCCTATAACCTTGCATATAAGTATAATTTTGAATATTCCGTGGTTTTCAACATGGTACTTTGGATAATG
E A K Q A K N P A S P Y N L A Y K Y N F E Y S V V F N M V L W I M
1110 1120 1130 1140 1150 1160 1170 1180 1190 1200
ATCGCCTTGGCCTTGGCTGTGATTATCACCTCTTACAATATTTGGAACATGGATCCTGGATATGATAGCATCATTTATAGGATGACAAACCAGAAGATTC
I A L A L A V I I T S Y N I W N M D P G Y D S I I Y R M T N Q K I
1210 1220 1230 1240 1250 1260 1270 1280 1290 1300
GAATGGATTGAATGTTACCTGTGCCAGAATTAGAAAAGGGGGTTGGAAATTGGCTGTTTTGTTAAAATATATCTTTTAGTGTGCTTTAAAGTAGATAGTA
R M D
1310 1320 1330 1340 1350 1360 1370 1380 1390 1400
TACTTTACATTTATAAAAAAAAATCAAATTTTGTTCTTTATTTTGTGTGTGCCTGTGATGTTTTTCTAGAGTGAATTATAGTATTGACGTGAATCCCACT
1410 1420 1430 1440 1450 1460 1470 1480 1490 1500
GTGGTATAGATTCCATAATATGCTTGAATATTATGATATAGCCATTTAATAACATTGATTTCATTCTGTTTAATGAATTTGGAAATATGCACTGAAAGAA
1510 1520 1530 1540 1550 1560 1570 1580 1590 1600
ATGTAAAACATTTAGAATAGCTCGTGTTATGGAAAAAAGTGCACTGAATTTATTAGACAAACTTACGAATGCTTAACTTCTTTACACAGCATAGGTGAAA
1610 1620 1630 1640 1650 1660 1670 1680 1690 1700
ATCATATTTGGGCTATTGTATACTATGAACAATTTGTAAATGTCTTAATTTGATGTAAATAACTCTGAAACAAGAGAAAAGGTTTTTAACTTAGAGTAGC
1710 1720 1730 1740 1750 1760 1770 1780 1790 1800
CCTAAAATATGGATGTGCTTATATAATCGCTTAGTTTTGGAACTGTATCTGAGTAACAGAGGACAGCTGTTTTTTAACCCTCTTCTGCAAGTTTGTTGAC
1810 1820 1830 1840 1850 1860 1870 1880 1890 1900
CTACATGGGCTAATATGGATACTAAAAATACTACATTGATCTAAGAAGAAACTAGCCTTGTGGAGTATATAGATGCTTTTCATTATACACACAAAAATCC
1910 1920 1930 1940 1950 1960 1970 1980 1990 2000
CTGAGGGACATTTTGAGGCATGAATATAAAACATTTTTATTTCAGTAACTTTTCCCCCTGTGTAAGTTACTATGGTTTGTGGTACAACTTCATTCTATAG
2010 2020 2030 2040 2050 2060 2070 2080 2090 2100
AATATTAAGTGGAAGTGGGTGAATTCTACTTTTTATGTTGGAGTGGACCAATGTCTATCAAGAGTGACAAATAAAGTTAATGATGATTCCAAAAAAAAAA
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