Table 1 of Hu, Mol Vis 2001; 7:14-19.


Table 1. CEM replacement plus undefined components

Ingredient               Amount per liter            Final Molarity
----------               ----------------            --------------

TAPSO-Free Acid          5.0 g                       1.90 x 10-2 M

ZnSO4·7H2O               0.15 mg                      5.00 x 10-7 M

CuSO4·5H2O               0.25 mg                      1.00 x 10-9 M

MnCl2·4H2O               9.9 x 10-5 mg                5.00 x 10-10 M

Selenious acid,
sodium salt
(Na2SeO3)                2.6 mg                       1.50 x 10-8 M

Hydrocortisone           10.0 mg                      2.80 x 10-8 M

Calf Serum,
heat-inactivated         1.0% by volume

Linoleic acid,
complexed with albumin   0.085 mg                     3.00 x 10-7 M

Insulin                  5.0 mg                       8.30 x 10-7 M

Transferrin              5.0 mg                       6.30 x 10-8 M

Putrescine·2HCl          0.3 mg                       1.86 x 10-6 M

L-Ascorbic acid          45.0 mg                      2.60 x 10-4 M

L-Glutamine              292.0 mg                     2.0 mM

Triiodothyronine         6.5 x 10-6 mg                1.00 x 10-11 M

Bovine Retina Extract*   0.5% by volume

L-Alanine                0.020 g                      2.2 x 10-4 M

L-Asparagine             0.025 g                      1.7 x 10-4 M

L-Aspartic Acid          0.020 g                      1.5 x 10-4 M

L-Glutamic Acid          0.030 g                      2.0 x 10-4 M

L-Glycine                0.025 g                      3.3 x 10-4 M

L-Proline                0.020 g                      1.7 x 10-4 M

L-Serine                 0.015 g                      1.4 x 10-4 M

Biotin                   0.10 mg                      4.1 x 10-7 M

Oxaloacetic              0.15 g                       1.1 x 10-3 M

Thymidine                0.30 mg                      1.2 x 10-6 M

Ferric Nitrate           0.10 mg                      2.5 x 10-7 M

*Bovine retinal extract is an RPE culture additive first introduced by Pfeffer et al. [20]. It is presumed to add important trophic factors to the RPE culture and is prepared by homogenizing 12 fresh bovine retinas per 100 ml CMF-BSS and stirring overnight in the cold in the dark. The mixture is cleared by centrifugation (12,000x g for 20 min).


Hu, Mol Vis 2001; 7:14-19 <http://www.molvis.org/molvis/v7/a3/>
©2001 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535