Figure 2 of
Hu, Mol Vis 2001;
Figure 2. Western blot analysis of Na,K ATPase in four culture media
Immunoblot Analysis of cultured human RPE cells with an antibody to Na,K ATPase. 15 mg of protein from RPE cell extracts was separated on an 8% Polyacrylamide gel and transferred to a nitrocellulose membrane. A single band of approximately 100 KDa was observed. This size corresponds to the a-1 isoform. The molecular weight standards are indicated on the right side. Expression levels were approximately equal under all culture conditions except for DMEM with low glucose.