Table 1 of
Morgans, Mol Vis 2001;
7:202-209.Table 1 of
Morgans, Mol Vis 2001;
7:202-209.
Table 1. PCR primer pairs
The PCR primers used to amplify rat a1F cDNA fragments (except for PCR product 8) were designed against the human a1F cDNA sequence (GenBank accession number for CACNA1F is AJ224874) [3]. The primer sequences for amplifying PCR product 8 are from rat, and their location is given relative to the rat sequence (GenBank accession number AF365975). All primer pairs were presumed to span at least one intron. The numbering of the PCR products is based on the product location in the 5' to 3' direction of the cDNA sequence.
a1F F = Forward primer, 5'-3' Location of Predicted
PCR primers on length
product R = Reverse primer, 5'-3' CACNA1F (bp)
------- ------------------------- ----------- ---------
1 F: GCAGATGGCCCTTCAATCTC 34-53 1523
R: GGCAGCGTGTACAGCTGGCC 1524-1505
2 F: GCAGATGGCCCTTCAATCTC 34-53 851
R: CCATGTCGGATCCCAGGAAG 885-866
3 F: CTTCCTGGGATCCGACATGG 866-885 691
R: GGCAGCGTGTACAGCTGGCC 1524-1505
4 F: TCCACACACTCCACCAGCAG 1407-1426 260
R: ATGGTCAACGTGTTGAGG 1666-1649
5 F: GGCCAGCTGTACACGCTGCC 1505-1524 1935
R: CCACGAAGATGTTCATCATG 3429-3410
6 F: ACAACCTGGCCAGTGGAGAT 2305-2324 1133
R: CCACGAAGATGTTCATCATG 3429-3410
7 F: CAGCCATGATGGCCCTGTTC 3250-3269 1186
R: CAGATCCTCTTGAATTCATC 4426-4407
8 F: TCTTCATGCTCTGTGCCT 4297-4314 1233
R: AGCCCTGCCTGGTCCTGA 5543-5526
9 F: CAGGCACCTATCATCGTG 5488-5505 466
R: TGGACGCAGGCCATCTCG 5953-5936
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