Figure 6. Double labeling for anti-a_1F(rat) and synaptotagmin.

Double labeling for a_1F and a synaptic vesicle marker in the outer retina demonstrates that a_1F is restricted to the photoreceptor cell bodies and is excluded from the synaptic terminals. Rat retina sections were doubled labeled for rat a_1F shown in green, and synaptotagmin (Tagmin) shown in red. The images were superimposed (right-most panel) to reveal areas of co-localization, which appear yellow. Abbreviations: IS, inner segments of photoreceptors; ONL, outer nuclear layer; OPL, outer plexiform layer.