Figure 5 of Schlamp, Mol Vis 2001; 7:192-201.


Figure 5. Analysis of Thy1 expression in optic nerve crush-resistant ganglion cells of Bax-/- mice

Analysis of Thy1 expression in retinal ganglion cells in Bax+/+ and Bax-/- mice after optic nerve crush. The change in Thy1 expression 1 week after optic nerve crush was monitored by in situ hybridization (A-D) and RPA analysis of equal amounts of total RNA (E). High magnification Nomarski images of the central retinal ganglion cell layer of a Bax+/+ mouse control eye (A) and experimental eye 1 week after crush (B). Like the CB6F1 mice, optic nerve crush causes a decrease in the number of Thy1 positive cells in the retina before there is noticeable cell loss. C: A similar view of the central retina from a control Bax-/- mouse, showing a typical increase in the number of ganglion cells and robust Thy1 staining. D: One week after optic nerve crush, Bax-/- mice also show a decrease in Thy1 staining. Size bar, 10 mm. E: The change in Thy1 expression observed by in situ hybridization was also monitored by RPA of total RNA. Bax-/- mice contain more Thy1 mRNA per mg of total RNA than wild-type littermates, but both groups of mice exhibit approximately 50% loss of the Thy1 mRNA 1 week after crush. No fragment was protected by the murine Thy1 probe in an equal mass of total RNA isolated from human retinoblastoma cells (hRB RNA).

(93 K)

Schlamp, Mol Vis 2001; 7:192-201 <http://www.molvis.org/molvis/v7/a27/>
©2001 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535