Figure 4. Western blot analysis of PEDF in human ocular tissues

Cornea (Co), neural retina (Re), and iris/ciliary body (I/C) were dissected from adult human eyes that had been frozen post mortem and stored at -80 °C. Protein from each tissue was loaded, along with the positive control protein ("+") obtained from transfected cells--see Methods. The blot was probed with pAb anti-PEDF ("poly"), stripped, and reprobed with mAb anti-PEDF ("mono"). Size (in kDa) is indicated to the left.