Figure 7. FRI of paxillin and phosphotyrosine in HTM cells treated with H-7

A-L: Digital microscopic analysis of HTM cells, double-labeled for paxillin (PAX; CY3) and phosphotyrosine (PT; FITC), before (0) and after 15 and 60 min (15'; 60') of treatment with 300 mM H-7. M-T: Cells treated with H-7 for 1 h and then incubated in medium without H-7 for 5 min or 20 min (60'+5'R; 60'+20'R). The left column shows the superimposed images of paxillin (red) and phosphotyrosine (green). The columns marked CY3 and FITC show the intensity of the respective labeling, using the spectrum scale presented under the FITC column. The FRI column depicts the CY3-to-FITC intensity ratio (scale shown under the column). Bar = 10 mm.