Figure 2. H-7-induced alteration and short-term recovery of cultured HTM cells

Phase contrast videography of cultured HTM cells. Cells were treated with 20, 100, or 300 mM H-7 and recorded at 2 h, then incubated with H-7-free medium and recorded at 30 min and 2 h. For each dose and time point, a different but representative field of cells was recorded, and no obvious cell loss was noted. Data for 2 h recovery are not shown. Bar = 80 mm.