Figure 1. H-7-induced alteration and long-term recovery of cultured HTM cells

Phase contrast videography of cultured HTM cells. Cells were treated with 20, 100, or 300 mM H-7 and recorded at 1, 6, 11 and 24 h, then incubated with H-7-free medium and recorded for another 24 h and 48 h. For each dose, the same field of cells was located and recorded at each time point, and no obvious cell loss was noted. Data for 6 h and 11 h treatment and 24 h recovery are not shown. Bar = 80 mm.