Figure 6 of Shaw, Mol Vis 2001; 7:6-13.


Figure 6. Cleavage of P347S mRNA

A: The protocol and logic of the RT-PCR reaction performed to monitor cleavage of the porcine P347S mRNA by the porcine P347S hammerhead ribozyme. RT-PCR fragments that represent wild type sequences are in black and RT-PCR fragments derived from the P347S mutant mRNA are in red. Complete digestion of the RT-PCR products with BanI leaves a 211 base-pair fragment that represents total P347S mutant mRNA. Complete digestion of the RT-PCR products with HgaI leaves a 211 base-pair fragment that represents wild type mRNA. B: Autoradiograph of a non-denaturing 6% polyacrylamide gel used to separate the BanI and HgaI digested RT-PCR products produced after the P347S hammerhead ribozyme cleavage reaction.

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Shaw, Mol Vis 2001; 7:6-13 <http://www.molvis.org/molvis/v7/a2/>
©2001 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535