Figure 3 of Timmers, Mol Vis 2001; 7:131-137.

Figure 3. Sections of rat eyes after injection with fluorescent microspheres

Rat eyes were injected with 2 ml of fluorescent microspheres followed by sampling of rats two h post-injection (A), 1 day post-injection (B), 3 days post-injection (C), and 7 days post-injection (D). The rats were sacrificed by carbon dioxide asphyxiation followed by cardiac perfusion with 4% paraformaldehyde. Cryostat sections of 15 mm were prepared and viewed under fluorescence and bright field microscopy. A massive retinal detachment was visible immediately following the subretinal injection of the fluorescent microsphere suspension (A). A cluster of beads remained visible after fixation and sectioning, although most of the injected beads were lost during the processing of the sections. The retina was completely re-attached one day after the injection (B). The fluorescent microspheres spread over an area that comprised approximately 50% to 60% of the total retinal area (B). In subsequent days (C and D), the retinal area with fluorescent beads remained constant.


(123 K)


(43 K)


(69 K)


(46 K)

Timmers, Mol Vis 2001; 7:131-137 <>
©2001 Molecular Vision <>
ISSN 1090-0535