Figure 2. Chaperone-like activity of a-crystallin target protein complexes

A: Thermal aggregation of bL-crystallin (0.2 mg/ml) at 60 °C, (solid triangles), or in the presence of 13.3 mM DTT, (open triangle); a-crystallin (0.02 mg/ml), (solid circles); a-crystallin-insulin complex (0.02 mg/ml), (open squares); 13.3 mM DTT + a-crystallin-insulin complex (0.02 mg/ml), (inverted solid triangles). Scatter profile of a-crystallin (0.02 mg/ml) + 13.3 mM DTT at 60 °C, (open circles). B: DTT-induced aggregation of insulin (0.2 mg/ml) at 37 °C, (solid circles), or in the presence of a-crystallin (0.1 mg/ml), (solid triangles); a-crystallin-bL-crystallin complex (0.1 mg/ml), (solid squares). Scatter profile of a-crystallin-bL-crystallin complex (0.1 mg/ml) + 13.3 mM DTT at 37 °C, (inverted open triangles).