Figure 2. Comparison of RT-PCR amplification of RNA isolated by different methods

The RNA was isolated by either a GITC-based method or a silica-based filter method from pigmented ocular tissues, iris and ciliary body (CB). A: Endogenous melanin was observed in the total RNA isolated using the GITC method but not from the filter method. B: Total RNA integrity showed no difference between the two methods on a denaturing agarose gel. C: Inhibition of RT-PCR in the RNA sample containing endogenous melanin was observed by differential amplification of the house-keeping gene GAPDH.