Figure 4. GFP expression with various promoter constructs

Various sized 5'-upstream Lim2 fragments were cloned into the green fluorescent protein (GFP) reporter vector and then transfected into chick embryo lens epithelial cells. A: Epithelial cells transfected with the empty vector (pro-). B: Epithelial cells transfected with vector containing the CMV promoter as a positive control (CMV). C: Epithelial cells transfected with Lim2 upstream fragment -140/+56 (-140). D: Epithelial cells transfected with Lim2 upstream fragment -160/+56 (-160). E: Epithelial cells transfected with Lim2 upstream fragment -200/+56 (-200). F: Epithelial cells transfected with Lim2 upstream fragment -400/+56 (-400).