Figure 2 of
Boyle, Mol Vis 2000;
Figure 2. Purity of a-crystallins resolved by Biosep-SEC-S4000 column chromatography
To determine if minor protein contaminants might be present in the a-crystallins fraction collected on the S-4000 column, 20 mg of the S-4000 a-crystallins fraction was injected into a C18 reverse phase column with a flow rate of 1 ml per min and a linear gradient of 5-60% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid, over 40 min. Two prominent peaks located at approximately 34.2 and 34.7 min correspond to the a-crystallins subunits B and A, respectively. No other peaks above baseline were present.